107 Pages
by
CRC Press
SOME ADVANTAGES OF CELL-BASED BIOSENSORS:
Isolation, purification, and immobilization of enzymes are often very difficult; these can be omitted with cell-based biosensors.
Some enzymes can lose their activity during isolation or immobilization; this risk is eliminated by the use of whole cells.
Enzymes in the cell's natural environment are usually extremely stable.
Multi-step enzyme reactions in intact cells can be used, making it possible to avoid the preparation of complicated artificial multi-enzyme systems.
Coenzymes and activators are often present in the cells, and thus, it is not necessary to add them into the system; the cell itself usually cares for their effective regeneration.
Introduction - Cell-Based Biosensors - Construction and Peculiarities of Basic Types of Cell-Based Biosensors - Properties of Cell-Based Biosensors - Causes of Cell-Based Biosensor Unselectivity - Methods of Selectivity Improvement of Cell-Based Biosensors - Practical Use of Cell-Based Biosensors - Conclusion and Future Prospects - Appendix - References
Biography
Jaroslav Racek
