1st Edition

Biotechnology of Plasma Proteins

By Roger L. Lundblad Copyright 2013
    460 Pages 20 B/W Illustrations
    by CRC Press

    The fractionation of human blood plasma can be considered to be a mature industry, with the basic technology, alcohol fractionation, dating back at least to the 1940s. Many of the products described in the current work have been approved biologics since the 1950s. The information gathered from the development of plasma proteins has proved vital to the development of recombinant therapeutic proteins.

    Discussing the role of plasma proteins in current biotechnology, Biotechnology of Plasma Proteins describes the protein composition of human plasma, the fractionation of plasma to obtain therapeutic proteins, and the analysis of these products. It delineates the path from plasma products to recombinant products, and highlights products from albumin, intravenous immunoglobins, and coagulation. It offers a comprehensive review of current techniques for the analysis of proteins including electrophoresis, chromatography, spectrophotometry, mass spectrometry, and updates not published since 1975.

    Key Topics

    Protein Composition of Plasma

    • Proteomic methods for plasma protein analysis
    • Plasma protein biomarkers
    • Validation of biomarkers
    • Assays for plasma biomarkers

    Methods for the Analysis of Protein Products

    • Assay development and validation
    • Electrophoresis
    • Chromatography
    • Immunoassay
    • Mass spectrometry
    • Raman spectroscopy

    Plasma Fractionation: Historical and Modern Methods

    • Development of Cohn alcohol fractionation
    • Industrial methods
    • Development of chromatographic methods

    Plasma Protein Products of Therapeutic Value

    • Albumin
    • Intravenous immunoglobulin
    • Coagulation products
    • Growth factors
    • Wound management

    Blood, Plasma, Cells, and Other Biological Fluids

    Plasma Fractionation and Plasma Protein Products

    Methods for the Analysis of Plasma and Plasma Protein Fractions

    Albumin
    Structure of Albumin
    In Vitro Chemical Modification of Albumin
    Albumin as a Carrier
    Ligand-Binding by Albumin
    Purification and Characterization of HSA for Clinical Use
    Biological Activity of Albumin
    Enzymatic Activity of Albumin
    Clinical Use of Albumin
    Albumin as Diagnostic/Biomarker
    Pharmacokinetics of Albumin
    Analytical Methods for Albumin Including Use of Albumin as Standard for Analytical Methods
    Albumin as Excipient
    Conclusion
    References

    Plasma Immunoglobulins

    Factor VIII and von Willebrand Factor
    von Willebrand Factor
    Economic Issues Impacting the Hemophilia Business
    Conclusions and Future Directions
    References

    Plasma Proteinase Inhibitors
    Antithrombin
    α1-Antitrypsin (α1-Antiprotease Inhibitor, SERPINA1)
    Heparin Cofactor II (SERPIND1)
    α2-Macroglobulin
    Tissue Factor Pathway Inhibitor
    α2-Antiplasmin
    C1-Inhibitor (C1-Esterase Inhibitor)
    Plasminogen Activator Inhibitor-1
    Protein C Inhibitor (Plasminogen Activator Inhibitor-3)
    References

    Vitamin K-Dependent Proteins

    Miscellaneous Plasma Proteins
    Fibrinogen
    Fibrin Sealant
    Autologous Fibrin Sealant
    Fibrinogen and Tissue Soldering
    Thrombin and Fibrin Foam
    Fibrinogen Plastics
    Thrombin
    Plasminogen and Plasmin
    Butyrylcholinesterase
    Fibronectin
    References

    Biography

    Roger L. Lundblad