Biomolecules and Organic Solvents discusses the behavior of enzymes and multi-enzyme complexes in organic solvents, in addition to the problem of water-protein interactions and the characteristics of water at interphases. It attempts to bridge the gap between the all water and the organic solvents systems from the point of view of enzyme structure and function. The mechanism of action of enzymes dispersed in anhydrous organic solvents and the biotechnological perspectives of the field are evaluated, and new information regarding the function and characteristics of complex multi-enzymatic systems and whole cells in organic solvents are examined. New developments regarding the nature of the water pool and interphases of reverse micelles and their enzyme kinetics are also explored. The characteristics and properties of enzymes trapped in reverse micelles are discussed throughout the book.
Biomolecules in Organic Solvents is essential reading for biochemists, molecular biologists, and others working in related fields.
Table of Contents
New Approaches in Studying Biomolecule-Water Interactions (H.F.J. Savage and J.L. Finney). Enzymes in Reverse Micelles Containing Phospholipids (Alberto Darszon and Liora Shoshani). The Microscopic Structure and Dynamics of Water at a Surface (Humberto Saint-Martin and Iván Ortega-Blake). Mechanistic Enzymology in Anhydrous Organic Solvents (Alan J. Russell, Sudipta Chatterjee, Igor Rapanovich, and James G. Goodwin, Jr.). Water and Enzymes in Organic Solvents (Georgina Garza-Ramos, D. Alejandro Fernández Velasco, M. Tuena de Gómez-Puyou, and A. Gómez-Pyou). The Optimization of Enzyme Catalysis in Organic Media (R.M.D. Verhaert, R. Hilhorst, A.J.W.G. Visser, and C. Veeger). Expression of Enzyme Activity in Reverse Micelles (F. Garcia-Carmona, R. Bru, and A. Sánchez-Ferrer). Enzyme Activity in Reverse Micelles: Roles of Diffusion and Extent of Hydration (Sérgio T. Ferreira and Enrico Grattan). Reversed Micelles: Recent Advances and Future Perspectives (Janos H. Fendler). Biological Electron Transport in Reverse Micellar Systems. From Enzymes to Cells (Edgardo Escamilla, Martha Contrertas, Laura Escobar, and Guadalupe Ayala). Solubilization of Microorganisms in Organic Solvents by Reverse Micelles (Nestor Pfammatter, Maria Famiglietti, Alejandro Hochköppler, and Pier Luigi Luisi).