Biomolecular Kinetics: A Step-by-Step Guide

Clive R. Bagshaw

© 2018 - CRC Press
Published October 3, 2017
Textbook - 446 Pages
ISBN 9781138066588 - CAT# K33435
Series: Foundations of Biochemistry and Biophysics

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  • Worked answers to exercises in the book are available to confirmed adopting instructors.
  • Introduces the analytical rate equations for basic one and two-step reactions relevant to the interactions of macromolecules with ligands.
  • Extends the analysis using the step-by-step approach of numerical integration and the "dice-rolling" approach of stochastic simulations.
  • Discusses the theory and practice of elucidating biomolecular mechanisms, including enzyme action, protein folding, protein-nucleic acid interactions and molecular motors.
  • Provides a comprehensive description of instruments and techniques for following transient reactions on a sub-second time scale and at the single-molecule level.
  • Illustrates the analysis of reaction records using computer simulation and fitting, using commercial software and freeware.


"a gem of a textbook which manages to produce a genuinely fresh, concise yet comprehensive guide"
–Mark Leake, University of York

"destined to become a standard reference…. Not just a ‘how to’ handbook but also an accessible primer in the essentials of kinetic theory and practice."
–Michael Geeves, University of Kent

"covers the entire spectrum of approaches, from the traditional steady state methods to a thorough account of transient kinetics and rapid reaction techniques, and then on to the new single molecule techniques"
–Stephen Halford, University of Bristol

This illustrated treatment explains the methods used for measuring how much a reaction gets speeded up, as well as the framework for solving problems such as ligand binding and macromolecular folding, using the step-by-step approach of numerical integration. It is a thoroughly modern text, reflecting the recent ability to observe reactions at the single-molecule level, as well as advances in microfluidics which have given rise to femtoscale studies. Kinetics is more important now than ever, and this book is a vibrant and approachable entry for anyone who wants to understand mechanism using transient or single molecule kinetics without getting bogged down in advanced mathematics.

Clive R. Bagshaw is Emeritus Professor at the University of Leicester, U.K., and Research Associate at the University of California at Santa Cruz, U.S.A.