Welcome to CRCPress.com! We have customized the Taylor & Francis India website to host CRC Press titles. Please choose www.TandFIndia.com to get the following benefits:
South Asia Editions of CRC Press titles with INR prices
Multiple options to purchase locally
All CRC Press products available
Your CRC Press login credentials will work on TandFIndia.com
Garland Science Website Announcement
The Garland Science website is no longer available to access and you have been automatically redirected to CRCPress.com.
All instructor resources (*see Exceptions) are now available on our Instructor Hub. Your GarlandScience.com instructor credentials will not grant access to the Hub, but existing and new users may request access here.
The student resources previously accessed via GarlandScience.com are no longer available to existing or new users.
The Second Edition of this bestseller brings together basic plant pathology methods published in diverse and often abstract publications. The Second Edition is updated and expanded with numerous new figures, new culture media, and additional methods for working with a greater number of organisms. Methods are easy to use and eliminate the need to seek out original articles. This reference allows for easy identification of methods appropriate for specific problems and facilities. Scientific names of pathogens and some of their hosts are updated in this edition. The book also acts as a research source providing more than 1,800 literature citations. The Second Edition includes chapters on the following:
Sterilization of culture apparatus and culture media
Culture of pathogens with detailed techniques for 61 fungi and selected bacteria
Long-term storage of plant pathogens
Detection and estimation of inoculum for 28 soilborne fungal pathogens and 5 bacterial genera-15 methods for airborne inoculum and 13 methods for seedborne pathogens
Establishment of disease and testing for disease resistance
Work with soil microorganisms
Table of Contents
Sterilization of Apparatus and Culture Media Heat Sterilization Gas Sterilization Filtration Culture of Pathogens Factors Affecting the Cultivation and Sporulation of Pathogenic Fungi Some Special Methods of Inducing Sporulation in Fungi Cultivation of Pathogens for Soil Infestation Other Sources of Inoculum Cultivation and Sporulation of Some Pathogens Long-Term Storage of Plant Pathogens Periodic Transfer Mineral Oil Drying Storage in Water Storage in Silica Gel Storage in Soil Freezing Preservation in Liquid Nitrogen Lyophilization and Vacuum Drying Detection and Estimation of Inoculum Soilborne Pathogens Detection and Estimation of Inoculum in the Air Detection of Pathogens Associated with Seeds Establishment of Disease and Testing for Resistance Factors Influencing Disease Establishment Root Inoculation Stem Inoculation Leaf Inoculation Inoculation of Cereal Inflorescences Seed Inoculation Inoculation with Bacteria Inoculation of Detached Plant Parts Field Inoculation Methods Some Special Hints Soil Microorganisms Isolation and Enumeration of Soil Organisms Observation of Soil Microorganisms in Situ Physiological Processes as an Estimate of Biological Activity in Soil Spore Germination in Soil and Soil Fungistasis Survival of Plant Pathogens in Soil Mycelial Growth in Soil Collection of Root and Seed Exudates Artificial Rhizosphere or Spermosphere Fungicide Evaluation Spore Germination Test Tests Based on Growth on Organisms In Vitro Transfer-Bioassay Techniques Laboratory Testing for Soil Fungicides Greenhouse Testing of Foliar Fungicides Greenhouse Testing of Fungicides for Control of Soilborne Pathogens Planning Field Experiments Seed Treatment with Chemicals Biological Control Collection and Testing of Soil Samples for Antagonists Isolation of Antibiotic-Producing Organisms Isolation and Testing of Lytic Organisms Isolation and Testing of Mycoparasitic Organisms Spore Perforating Amoebae Isolation of Antagonists from Leaves Testing Antagonists for Biological Control Bright-Field Microscopy Techniques Mounting and Staining of Fungi for Microscopic Examination Staining of Fungal Nucelli Mounting Spores for Viewing from Different Positions Immobilization of Fungus Spores in Water Mounts for Microscopic Examination Staining of Bacteria Cements for Sealing Mounts Slide Culture Techniques and Preparation of Permanent Mounts Preparing Agar-Colony Mounts and Dry Reference Cultures Examination of Host-Parasite Relationship Using Bright-Field Microscopy Appendices Culture Media and Their Formulas Plant Nutrient Solutions Solutions to Maintain Constant Humidity in a Closed Atmosphere Drying Agents pH Indicator Dyes Approximate Figures for Concentrated Acids and Bases Preservation of Museum Specimens Collection, Preparation, and Mailing of Cultures and Specimens Glassware Cleaning Solutions Index