A Practical Guide to Assay Development and High-Throughput Screening in Drug Discovery

Taosheng Chen

December 21, 2009 by CRC Press
Reference - 291 Pages - 83 B/W Illustrations
ISBN 9781420070507 - CAT# 70509
Series: Critical Reviews in Combinatorial Chemistry

USD$199.95

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Features

  • Integrates the knowledge of experts in biology, chemistry, informatics, instrumentation, automation, technology development, and project management
  • Enables researchers in industrial and academic settings to find the most efficient way to carry a project from start to finish
  • Identifies critical targets that are of relevance to drug discovery
  • Discusses the components of the HTS process and provides step-by-step instructions
  • Examines emerging technologies and systems

 

    Summary

    The development of suitable assays, the integration of appropriate technology, and the effective management of the essential infrastructure are all critical to the success of any high-throughput screening (HTS) endeavor. However, few scientists have the multidisciplinary experience needed to control all aspects of an HTS drug discovery project. A Practical Guide to Assay Development and High-Throughput Screening in Drug Discovery integrates the experience of diverse experts who offer fundamental and practical guidance across numerous situations.

    The book first discusses assay developments for important target classes such as protein kinases and phosphatases, proteases, nuclear receptors, G protein-coupled receptors, ion channels, and heat shock proteins. It next examines assay developments for cell viability, apoptosis, and infectious diseases. The contributors explore the application of emerging technologies and systems, including image-based high content screening, RNA interference, and primary cells. Finally, they discuss the essential components of the integrated HTS process, such as screening automation, compound library management, the screening of natural products from botanical sources, and screening informatics.

    Designed to motivate researchers to bring further advances to the field, this volume provides practical guidance on how to initiate, validate, optimize, and manage a bioassay intended to screen large collections of compounds. Drawing on the knowledge from experts actively involved in assay development and HTS, this is a resource that is both comprehensive and focused.