Life at the Nanoscale

Life at the Nanoscale: Atomic Force Microscopy of Live Cells

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ISBN 9789814267960
Cat# N10435

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Features

  • Published in color with numerous illustrations
  • Comprehensive overview of the use of atomic force microscopy and related scanning probe techniques for analyzing cells and membranes, going from the basics to the applications side

Summary

Proceeding from basic fundamentals to applications, this volume provides a comprehensive overview of the use of AFM and related scanning probe microscopies for cell surface analysis. It covers all cell types, from viruses and protoplasts to bacteria and animal cells. It also discusses a range of advanced AFM modalities, including high-resolution imaging, nanoindentation measurements, recognition imaging, and single-molecule and single-cell force spectroscopy. The book covers methodologies for preparing and analyzing cells and membranes of all kinds and highlights recent examples to illustrate the power of AFM techniques in life sciences and nanomedicine.

Table of Contents

Observing the Nanoscale Organization of Model Biological Membranes by Atomic Force Microscopy
Pierre-Emmanuel Milhiet and Christian Le Grimellec
High-Resolution Atomic Force Microscopy of Native Membranes
Nikohy Buzhynskyy, Lu-Ning Liu, Ignacio Casuso and Simon Scheuring
Microbial Cell Imaging Using Atomic Force Microscopy
Mitchel J. Doktycz, Claretta J. Sullivan, Ninell Pollas Mortensen and David P. Allison
Resolving the High-Resolution Architecture, Assembly and Functional Repertoire of Bacterial Systems by in vitro Atomic Force Microscopy
Alexander J. Malkin
Understanding Cell Secretion and Membrane Fusion Processes on the Nanoscale Using the Atomic Force Microscope
Bhanu P. Jena
Nanophysiology of Cells, Channels and Nuclear Pores
Hermann Schillers, Hans Oberleithner and Victor Shahin
Topography and Recognition Imaging of Cells
Lilia Chtcheglova, Linda Wildling and Peter Hinterdorfer
High-Speed Atomic Force Microscopy for Dynamic Biological Imaging
Takayuki Uchihashi and Toshio Ando
Near-Field Scanning Optical Microscopy of Biological Membranes
Thomas S. van Zanten and Maria F. Garcia-Parajo
Quantifying Cell Adhesion Using Single-Cell Force Spectroscopy
Anna Taubenberger, Jens Friedrichs and Daniel J. Mutter
Probing Cellular Adhesion at the Single-Molecule Level
Félix Rico, Xiaohui Zhang and Vincent T. Moy
Mapping Membrane Proteins on Living Cells Using the Atomic Force Microscope
Atsushi Ikai and Rehana Afrin
Probing Bacterial Adhesion Using Force Spectroscopy
Terri A. Camesano
Force Spectroscopy of Mineral-Microbe Bonds
Brian H. Lower and Steven K. Lower
Single-Molecule Force Spectroscopy of Microbial Cell Envelope Proteins
Claire Verbelen, Vincent Dupres, David Alsteens, Guillaume Andre and Yves F. Dufrêne
Probing the Nanomechanical Properties of Viruses, Cells and Cellular Structures
Sandor Kasas and Giovanni Dietler
Label-Free Monitoring of Cell Signalling Processes Through AFM-Based Force Measurements
Charles M. Cuerrier, Elie Simard, Charles-Antoine Lamontagne, Julie Boucher, Yannick Miron and Michel Grandbois
Investigating Mammalian Cell Nanomechanics with Simultaneous Optical and Atomic Force Microscopy
Yaron R. Silberberg, Louise Guolla and Andrew E. Felling
The Role of Atomic Force Microscopy in Advancing Diatom Research into the Nanotechnology Era
Michael]. Higgins and Richard Wetherbee
Atomic Force Microscopy for Medicine
Shivani Sharma and James K. Gimzewski

Editor Bio(s)

Yves Dufrêne received his engineering degree in chemistry and bioindustries (in 1991) and a PhD degree in biophysical chemistry (in 1996) at the Université catholique de Louvain (UCL, Belgium). After a postdoc at the Naval Research Laboratory (Washington DC, USA), he became research associate (2000) of the Belgian National Fund for Scientific Research and lecturer in physical chemistry and nanobiotechnology at UCL. He is currently the head of the Laboratory of Chemistry of Interfaces.

Editorial Reviews

"Atomic force microscopy (AFM) began as a topographical surface imaging technique, but its ability to measure the tiny forces experienced and exerted by cells and the molecules therein has made it an indispensible method for biological research. These forces are central to cell function, and no optical microscopy technique yet surpasses AFM methods for investigating these physical processes at the level of molecules and cells. Now, in this book devoted to the use of AFM in biology, leading scientists using these methods in their own research discuss the great variety of biological applications that have been developed and the many unique discoveries they have allowed. Accessible but informative descriptions and stunning images offer biologists an excellent introduction to this powerful but underexploited methodology. Any biologist considering the use of AFM in their own research is certain to find inspiration in this unique resource."
—Dr. Daniel Evanko, Chief Editor, Nature Methods

"Life at the Nanoscale provides a state-of-the-art overview about how atomic force microscopy (AFM) can be used to image living cells, to probe their attachment to substrates or to other cells, to measure their mechanical properties, and to assess the forces in single-molecule interactions. Experts in the field have contributed chapters that summarize their hands-on experience in concise, and hence most useful, reviews. So many insightful experiments have been achieved over the last decade, and many of them are now presented in this resourceful book, which comes timely for students and experts who have an interest in the interdisciplinary field of cell biology and in the application of tools that address single molecules."
—Prof. Andreas Engel, Case Western Reserve University, USA