Lipid Analysis and Lipidomics: NewTechniques and Applications
Summary
New methods for the analysis of edible oils, fats, and cellular lipids have recently been developed, presented at scientific meetings, and published in peer-reviewed journals. These methods apply to biological and food matrices, edible oils and fats, as well as cellular fats of pathogenic bacteria and spores, and will cover many research applications in lipidomics, food analysis, food safety, food security, and counter-terrorism. This text offers the lipid analyst essential analytical tools in the fields of chromatography, mass spectrometry, spectroscopy, magnetic resonance, and chemometrics. It also serves as a reference for recent developments in the rapidly evolving field of lipid methodologies.
Table of Contents
OVERVIEW
Techniques and Applications in Lipid Analysis
MASS SPECTRAL TECHNIQUES/LIPIDOMICS
An Overview of Modern Mass Spectrometry Methods in the Toolbox of Lipid Chemists and Biochemists
Global Cellular Lipidome Analyses by Shotgun Lipidomics Using Multidimensional Mass Spectrometry
LC/MS and Chiral Separation
LC/MS and Lipid Oxidation
Structural Analysis of Unsaturated Fatty Acid Methyl Ester Isomers with Acetonitrile Covalent Adduct Chemical Ionization
CHROMATOGRAPHIC TECHNIQUES
Recent Advances in Silver-Ion HPLC Utilizing Acetonitrile in Hexane as Solvent: Temperature Effects on Lipid Elution Orders/Resolution
Analysis of trans-18:1 Fatty Acids by Silver Ion HPLC
High-Performance Size-Exclusion Chromatography for Lipid Analysis in Organic Media
Lipid Separations Using Packed-Column Supercritical Fluid Chromatography
TLC-FID with Special Reference to Marine Lipids and Other High-Molecular-Weight Organic Compounds
Fast GC for Cellular FAME Analysis of Bacteria
VIBRATIONAL SPECTROSCOPIC TECHNIQUES
Use of Cellular Fatty Acids to Identify Food-Borne Pathogens by Infrared Spectroscopy and Capillary Gas Chromatography
Application of FT-NIR for Rapid Determination of the Trans Fatty Acid Composition in Fats and Oils
Infrared Spectroscopy and Partial Least Square Calibration in the Simultaneous Quantification of Isolated trans and Conjugated Linoleic Acids
Investigation of Protein-Lipid Interactions by Vibrational Spectroscopy
APPLICATIONS
Fat Replacers: An Overview
Phospholipids: Structures and Physicochemical Activities
Waxes and Sterols: Structures and Chemistry
