1st Edition
Isolation, Identification and Characterization of Allelochemicals/ Natural Products
A lot of allelopathy research has been done in various fields of Agricultural and Plant Sciences. However, there is no compilation of various research methods used. To make available the standard methods for conducting allelopathy research independently, this multi-volume book has been planned. Since allelopathy is multi-disciplinary area of research, hence, volumes have been planned for each discipline. This book has been written to provide information about all aspects of Allelochemicals/Natural Products. The book is divided in 3 sections Sample Collection, Handling and Storage; Isolation, Identification and Structural Elucidation and Biological Activity of Natural Products. This book will serve as ready reference in the laboratory or class room to undergraduate and graudate students and Organic Chemists for Isolation, Identification and Characterization of Allelochemicals/Natural Products. The editors have tried to provide appropriate solutions to the problems of Isolation, Identification and Characterization of Allelochemicals/Natural Products. The users of this book can select suitable methods, according to the available facilities.
Preface
SECTION I. Sample Collection, Handling and Storage
Allelopathy and Allelochemicals S. S. Narwal and D.A. Sampietro
Soil, Air and Water Samples D.A. Sampietro, J.R. Soberón, M.A. Sgariglia, E.N. Quiroga and M.A. Vattuone
Plant Sampling and Sample Preparation M.A. Vattuone, D.A. Sampietro, J.R. Soberón, M.A. Sgariglia and E.N. Quiroga
SECTION II. Isolation, Identification and Structural Elucidation
Colorimetric Reactions D.A. Sampietro, M.A. Sgariglia, J.R. Soberón, E.N. Quiroga and M.A. Vattuone
TLC and PC S.L. Debenedetti
HPLC and LC- MS E.G. Wilson
Chromatography and Spectroscopy of Alkaloids S.R. Leicach, H.D. Chludil and M.A. Yaber Grass
GC and GC-MS of Terpenoids M.L. López, J.S. Dambolena, M.P. Zunino and J.A. Zygadlo
GC and GC-MS for Non-volatile Compounds M.S. Maier
Spectrometry: Ultraviolet and Visible Spectra C.B. Colloca and V.E. Sosa
Spectrometry: Infrared Spectra A. del Valle Pacciaroni and V.E. Sosa
NMR Pedro C. Rossomando
SECTION III. Biological Activity of Natural Products
Bioassays with Whole Plants and Plant Organs D.A. Sampietro, E.N. Quiroga, J.R. Soberón,
M.A. Sgariglia and M.A. Vattuone
Bioassays on Plants: Plant Cells and Organelles O. Ferrarese-Filho, M.L.L. Ferrarese and W.D. dos Santos
Bioassays on Microorganisms: Antifungal and Antibacterial Activities M.A. Sgariglia, J.R. Soberón, D.A. Sampietro, E.N. Quiroga and M.A. Vattuone
Bioassays for Antioxidant, Genotoxic, Mutagenic and Cytotoxic Activities J.R. Soberón, M.A. Sgariglia, D.A. Sampietro, E.N. Quiroga and M.A. Vattuone
Bioassays: Inhibitors of Insect Chitin-degrading Enzymes T. Nitoda and H. Kanzaki
Bioassays: Insect Behaviour, Development and Survival M.C. Carpinella, M. T. Defagó, G. Valladares and S.M. Palacios
SECTION IV. Appendices
Abbreviations
Chemical Formulae and Molecular Weight of Solvents and Reagents
III. Molecular Weight of Organic Compounds
Index
About the Editors
Biography
Sampietro, Diego A ; Catalan, Cesar A.N.; Vattuone, Marta A.
