Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery

Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery

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ISBN 9781574445725
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Features

  • Reviews basic principles of micromachining and fluid flow and control for lab-on-a-chip technology
  • Emphasizes analytical applications of microfluidic technology
  • Includes information key to cell, nucleic acid, protein, and genetic analyses, including DNA sequencing
  • Adapts traditional principles of operations for the size of a chip, optimizing the miniaturization advantage and favorable scaling down
  • Gleans significant and detailed references from the vast literature on each topic for the more experienced researcher
  • Contains figures that represent novel designs and breakthroughs in the field, tables that summarize related materials, and problem sets that test comprehension of the material
  • Summary

    The microfluidic lab-on-a-chip allows scientists to conduct chemical and biochemical analysis in a miniaturized format so small that properties and effects are successfully enhanced, and processes seamlessly integrated. This microscale advantage translates into greater sensitivity, more accurate results, and better information.

    Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery focuses on all aspects of the microfluidic lab-on-a-chip technologies and offers an overview of the available technology, its limitations, and its breakthroughs over the years. It emphasizes analytical applications of microfluidic technology and offers in-depth coverage of micromachining methods, microfluidic operations, chemical separations, sample preparation and injection methods, detection technology, and various chemical and biological analyses. Other topics of interest include the use of polymeric chips, fluid flow valve and control, single-cell analysis, DNA and RNA amplification techniques, DNA hybridization, immunoassays and enzymatic assays.

    Originally conceived as a single chapter published in Ewing's Analytical Instrumentation, this book is a gateway to the vast literature and conference proceedings on the topic. Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery expands upon its roots to present a comprehensive treatment of microfluidic lab-on-a-chip methods and applications for novices and advanced researchers alike.

    Table of Contents

    Introduction

    Micromachining Methods
    Micromachining of Silicon
    Micromachining of Glass
    Micromachining of Fused Quartz (or Fused Silica)
    Micromachining of Polymeric Chips
    Metal Patterning
    World-to-Chip Interface

    Microfluidic Flow
    Liquid Pumping Methods
    Microfluidic Flow Control


    Sample Introduction
    Electrokinetic Injection
    Hydrodynamic Injection
    Other Sample Injection Methods

    Sample Pre-concentration
    Sample Stacking
    Extraction
    Porous Membrane
    Other Pre-concentration Methods

    Separation
    Gas Chromatography (GC)
    Capillary Electrophoresis (CE)
    Chromatographic Separations
    Coupled Separations

    Detection Methods
    Optical Detection Methods
    Electrochemical (EC) Detection
    Mass Spectrometry (MS)
    Other Detection Methods

    Applications to Cellular/Particle Analysis
    Retention of Cells and Particles
    Studies of Cells in a Flow
    Other Cell Operations

    Applications to Nucleic Acids Analysis
    Nucleic Acids Extraction and Purification
    Nucleic Acids Amplification
    DNA Hybridization
    Other Nucleic Acid Applications

    Applications to Protein Analysis
    Immunoassay
    Protein Separation
    Enzymatic Assays

    Appendix

    Problem Sets
    Introduction
    Micromachining
    Microfluidic Flow
    Sample Introduction
    Sample Preconcentration
    Separation
    Detection
    Cellular Analysis
    Nucleic Acid Analysis
    Protein Analysis
    References
    Glossary

    Editorial Reviews

    "…truly an awesome book…This well-documented book with a multitude of references: 1/178 references are integrated throughout the book…well-written and compares well to other science books of its kind…a great book to learn about instrument design, with numerous pictures and illustrations making the text much easier to understand. Good pictures are something that is lacking in all too many science books."
    -Gary S. Vardon, Salt Lake Tribune, in Journal of Materials

    "In fact I can say that it is an excellent book, well thought out and written."

    – P. Myers, in Chromatographia, June 2007, Vol. 65