The Fluorescent Protein Revolution
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Features
- Discusses major advancements in the development of novel imaging techniques
- Gives up-to-date information on state-of-the-art imaging methods
- Describes the applications of fluorescent probes in a variety of different optical techniques
Summary
This reference discusses major advances in the development of novel imaging techniques that exploit new probes. It describes the photophysical properties of new fluorescent probes, particularly the important red fluorescent probes from corals. The book gives a detailed account of state-of-the-art imaging methods that exploit the characteristics of new probes. It also illustrates the applications of fluorescent probes in a variety of optical techniques, including superresolution microscopy. Many figures throughout the text further clarify concepts.
Table of Contents
HISTORY AND PERSPECTIVES
Introduction to Fluorescent Proteins; Ward
Development of Aequorea victoria Fluorescent Proteins; Miyawaki
The Quest for New Fluorescent Proteins from Corals; Matz, Lukyanov,and Wiednmann
PHOTOPHYSICAL PROPERTIES OF FLUORESCENT PROTEINS
Lessons Learned from the Structure of Aequorea Victoria Fluorescent Proteins; Remington
Development of Optimized Fluorescent Proteins; Waldo
Development of New Colors from Coral Fluorescent Proteins; Campbell
The Search for the Grail: Optimal Red Fluorescent Proteins; Davidson, Verkhusha, and Wiedenmann
Optical Highlighters; Lukyanov, Chudakov, and Davidson
Far Red and Near Infared Fluorescent Proteins; Paul Steinbach, Shu, and Lin
APPLICATIONS
Genetically Encoded Fluorescent Proteins and FRAP; McNally
Optical Highlighters; Lippincott-Schwartz and Patterson
Biosensor Probes; Zhang
Probes for FRET and FLIM; Day
Superresolution Techniques; Davidson
Whole Animal Imaging with Red, Near-Infrared Probes; Tsien
Future Prospects; Davidson and Day
