Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics

Free Standard Shipping

Purchasing Options

ISBN 9781439861677
Cat# K12851



SAVE 20%

eBook (VitalSource)
ISBN 9781439861684
Cat# KE13031



SAVE 30%

eBook Rentals

Other eBook Options:


  • Provides a comprehensive treatment of fluorescence lifetime spectroscopy and imaging using both endogenous and exogenous probes
  • Discusses the fundamentals, state-of-the-art techniques, and their current applications in tissue diagnostics for research and clinical applications
  • Written by the leading experts in this field, which has advanced as a result of tremendous progress in optical and electronics technologies, including pulsed lasers and fast electronics, detectors and imaging cameras
  • Offers a survey of instrumentation and analysis of fluorescence lifetime data, including global analysis and computational approaches
  • Covers the wide range of clinical applications in oncology, cardiovascular, metabolism, dermatology, tissue engineering, and ophthalmology, as well as photodynamic therapy and tomographic FLIM of animals


During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics explores the remarkable advances in time-resolved fluorescence techniques and their role in a wide range of biological and clinical applications.

Broadly accessible, the book captures the state-of-the-art of fluorescence lifetime metrology and imaging and provides current perspectives on their applications to biomedical studies of intact tissues and medical diagnosis. The text introduces these techniques within the wider context of fluorescence spectroscopy and describes basic principles underlying current instrumentation for fluorescence lifetime imaging and metrology (FLIM). It also covers the wide range of methods, including single channel (point) spectroscopy, fluorescence lifetime imaging microscopy, and single- and multi-photon excitation.

Edited by pioneers in this field, with contributions from leading experts, the book includes an overview of complementary techniques that help researchers beginning FLIM research. It offers a comprehensive treatment of fundamental principles, instrumentation, analytical methods, and applications. It also provides an overview of the label-free contrast available from lifetime measurements of tissue autofluorescence and the prospects for exploiting this for clinical applications and biomedical research including drug discovery.

Table of Contents

Overview of Fluorescence Measurements and Concepts
Overview of Fluorescence Lifetime Imaging and Metrology, Daniel S. Elson, Laura Marcu, and Paul M. W. French
Photophysics of Fluorescence, Klaus Suhling
Tissue Fluorophores and Their Spectroscopic Characteristics, Alzbeta Chorvatova and Dusan Chorvat

Principles of Fluorescence Lifetime Instrumentation
Pulse Sampling Technique, Diego R. Yankelevich, Daniel S. Elson, and Laura Marcu
Single-Point Probes for Lifetime Spectroscopy: Time-Correlated Single-Photon Counting Technique, Christopher Dunsby and Paul M. W. French
Optical Instrumentation Design for Fluorescence Lifetime Spectroscopy and Imaging, Peter T. C. So, Heejin Choi, Christopher J. Rowlands, and Vijay R. Singh
Fluorescence Lifetime Imaging Techniques: Frequency-Domain FLIM, John Paul Eichorst, Kai wen Teng, and Robert M. Clegg
Fluorescence Lifetime Imaging Techniques: Time-Gated Fluorescence Lifetime Imaging, James McGinty, Christopher Dunsby, and Paul M. W. French
Fluorescence Lifetime Imaging Techniques: Time-Correlated Single-Photon Counting, Wolfgang Becker

Analysis of Fluorescence Lifetime Data
The Phasor Approach to Fluorescence Lifetime Imaging: Exploiting Phasor Linear Properties, Michelle A. Digman and Enrico Gratton
Analysis of Time-Domain Fluorescence Measurements Using Least-Squares Deconvolution, Jing Liu, Daniel S. Elson, and Laura Marcu
Global Analysis of FLIM-FRET data, Hernán E. Grecco and Peter J. Verveer
Fluorescence Lifetime Imaging in Turbid Media, Vadim Y. Soloviev, Teresa M. Correia, and Simon R. Arridge

Tissue Autofluorescence Lifetime Spectroscopy and Imaging: Applications
Oncology Applications: Optical Diagnostics of Cancer, Alzbeta Chorvatova and Dusan Chorvat
Oncology Applications: Brain, Pramod V. Butte, Adam N. Mamelak, and Laura Marcu
Oncology Applications: Skin Cancer, Rakesh Patalay, Paul M. W. French, and Christopher Dunsby
Oncology Applications: Gastrointestinal Cancer, Sergio Coda, Paul M. W. French, and Christopher Dunsby
Oncology Applications: Intraoperative Diagnosis of Head and Neck Carcinoma, D. Gregory Farwell and Laura Marcu
Fluorescence Lifetime Techniques in Cardiovascular Disease Diagnostics, Jennifer E. Phipps, Yang Sun, and Laura Marcu
Ophthalmic Applications of FLIM, Dietrich Schweitzer
Fluorescence Lifetime Imaging Applications in Tissue Engineering, Bernard Y. Binder, J. Kent Leach, and Laura Marcu

Fluorescence Lifetime Imaging Based on Exogenous Probes
Tomographic Fluorescence Lifetime Imaging, Anand T. N. Kumar
Photosensitizers and PDT, Rinaldo Cubeddu, Paola Taroni, and Gianluca Valentini
Fluorescence Lifetime Imaging of Ions in Biological Tissues, Christoph Biskup and Thomas Gensch

Editorial Reviews

"This highly recommended comprehensive volume is a good resource for investigators who wish to apply these techniques. It is a self-contained book in which the physics and the analytical methods are carefully worked out in detail. … The discussions of fluorescence, the design and use of lifetime instrumentation, the various methods to analyze the data, and the biomedical applications are all current and well-illustrated."
Optics & Photonics News (OPN), October 2014

"… a timely and comprehensive review of the state of the art by internationally leading experts in the field. It provides excellent coverage of the basic principles, as well as a thorough appraisal of the latest methods and applications. The book represents a major resource for researchers, students, and technologists."
—Jem Hebden, Ph.D., Professor and Head, Department of Medical Physics and Bioengineering, University College London

"This book provides comprehensive coverage on key aspects of fluorescence lifetime imaging, an emerging technique for life sciences and clinical diagnosis. The instrumentation and analysis sections include both well-known techniques and recent developments."
—Dr. Qiyin Fang, Associate Professor of Engineering Physics, McMaster University