Biotechnology of Plasma Proteins

Biotechnology of Plasma Proteins

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Features

  • Describes the protein composition of plasma
  • Presents methods for the analysis of protein products
  • Includes information on mass spectrometry and Raman spectroscopy
  • Gives a description of plasma protein products of therapeutic value
  • Discusses the history and current methods of plasma fractionation

Summary

The fractionation of human blood plasma can be considered to be a mature industry, with the basic technology, alcohol fractionation, dating back at least to the 1940s. Many of the products described in the current work have been approved biologics since the 1950s. The information gathered from the development of plasma proteins has proved vital to the development of recombinant therapeutic proteins.

Discussing the role of plasma proteins in current biotechnology, Biotechnology of Plasma Proteins describes the protein composition of human plasma, the fractionation of plasma to obtain therapeutic proteins, and the analysis of these products. It delineates the path from plasma products to recombinant products, and highlights products from albumin, intravenous immunoglobins, and coagulation. It offers a comprehensive review of current techniques for the analysis of proteins including electrophoresis, chromatography, spectrophotometry, mass spectrometry, and updates not published since 1975.

Key Topics

Protein Composition of Plasma

  • Proteomic methods for plasma protein analysis
  • Plasma protein biomarkers
  • Validation of biomarkers
  • Assays for plasma biomarkers

Methods for the Analysis of Protein Products

  • Assay development and validation
  • Electrophoresis
  • Chromatography
  • Immunoassay
  • Mass spectrometry
  • Raman spectroscopy

Plasma Fractionation: Historical and Modern Methods

  • Development of Cohn alcohol fractionation
  • Industrial methods
  • Development of chromatographic methods

Plasma Protein Products of Therapeutic Value

  • Albumin
  • Intravenous immunoglobulin
  • Coagulation products
  • Growth factors
  • Wound management

Table of Contents

Blood, Plasma, Cells, and Other Biological Fluids

Plasma Fractionation and Plasma Protein Products

Methods for the Analysis of Plasma and Plasma Protein Fractions

Albumin
Structure of Albumin
In Vitro Chemical Modification of Albumin
Albumin as a Carrier
Ligand-Binding by Albumin
Purification and Characterization of HSA for Clinical Use
Biological Activity of Albumin
Enzymatic Activity of Albumin
Clinical Use of Albumin
Albumin as Diagnostic/Biomarker
Pharmacokinetics of Albumin
Analytical Methods for Albumin Including Use of Albumin as Standard for Analytical Methods
Albumin as Excipient
Conclusion
References

Plasma Immunoglobulins

Factor VIII and von Willebrand Factor
von Willebrand Factor
Economic Issues Impacting the Hemophilia Business
Conclusions and Future Directions
References

Plasma Proteinase Inhibitors
Antithrombin
α1-Antitrypsin (α1-Antiprotease Inhibitor, SERPINA1)
Heparin Cofactor II (SERPIND1)
α2-Macroglobulin
Tissue Factor Pathway Inhibitor
α2-Antiplasmin
C1-Inhibitor (C1-Esterase Inhibitor)
Plasminogen Activator Inhibitor-1
Protein C Inhibitor (Plasminogen Activator Inhibitor-3)
References

Vitamin K-Dependent Proteins

Miscellaneous Plasma Proteins
Fibrinogen
Fibrin Sealant
Autologous Fibrin Sealant
Fibrinogen and Tissue Soldering
Thrombin and Fibrin Foam
Fibrinogen Plastics
Thrombin
Plasminogen and Plasmin
Butyrylcholinesterase
Fibronectin
References