Adaptive Optics for Biological Imaging
Purchasing Options
Features
- Provides the only comprehensive treatment of the principles, methods, and applications of adaptive optics in microscopy for biological imaging
- Focuses on biological imaging and synthesizes research from the first decade of this emerging field
- Takes an interdisciplinary perspective, making the material accessible to nonspecialists without a background in applied optics
- Shows how to use adaptive optics in the microscopy of biological samples to improve image quality and provide deeper tissue imaging
- Discusses how the wide availability of MEMS components has enabled applications of adaptive optics in biological imaging
- Includes more than 240 illustrations, plus a 12-page color insert
Summary
Adaptive Optics for Biological Imaging brings together groundbreaking research on the use of adaptive optics for biological imaging. The book builds on prior work in astronomy and vision science. Featuring contributions by leaders in this emerging field, it takes an interdisciplinary approach that makes the subject accessible to nonspecialists who want to use adaptive optics techniques in their own work in biology and bioengineering.
Organized into three parts, the book covers principles, methods, and applications of adaptive optics for biological imaging, providing the reader with the following benefits:
- Gives a general overview of applied optics, including definitions and vocabulary, to lay a foundation for clearer communication across disciplines
- Explains what kinds of optical aberrations arise in imaging through various biological tissues, and what technology can be used to correct for these aberrations
- Explores research done with a variety of biological samples and imaging instruments, including wide-field, confocal, and two-photon microscopes
- Discusses both indirect wavefront sensing, which uses an iterative approach, and direct wavefront sensing, which uses a parallel approach
Since the sample is an integral part of the optical system in biological imaging, the field will benefit from participation by biologists and biomedical researchers with expertise in applied optics. This book helps lower the barriers to entry for these researchers. It also guides readers in selecting the approach that works best for their own applications.
Table of Contents
Principles
Principles of Wave Optics
Donald T. Gavel
Principles of Geometric Optics
Joel Kubby
Theory of Image Formation
Michael Schwertner
Methods
Aberrations and Benefit of Their Correction in Confocal Microscopy
Michael Schwertner
Specimen-Induced Geometrical Distortions
Michael Schwertner
Simulation of Aberrations
Michael Schwertner
Overview of Adaptive Optics in Biological Imaging
Elijah Y.S. Yew and Peter T.C. So
Wavefront Correctors
Joel Kubby
Adaptive Optics System Alignment and Assembly
Diana C. Chen
Applications: Indirect Wavefront Sensing
Sensorless Adaptive Optics for Microscopy
Martin J. Booth and Alexander Jesacher
Implementation of Adaptive Optics in Nonlinear Microscopy for Biological Samples Using Optimization Algorithms
John M. Girkin
AO Two-Photon Fluorescence Microscopy Using Stochastic Parallel Descent Algorithm with Zernike Polynomial Basis
Yaopeng Zhou
Pupil-Segmentation-Based Adaptive Optics for Microscopy
Na Ji and Eric Betzig
Applications: Direct Wavefront Sensing
Coherence-Gated Wavefront Sensing
Jonas Binding and Markus Rückel
Adaptive Optics in Wide-Field Microscopy
Peter Kner, Zvi Kam, David A. Agard, and John Sedat
Biological Imaging and Adaptive Optics in Microscopy
Elijah Y.S. Yew, Jae Won Cha, Jerome Ballesta, and Peter T.C. So
Adaptive Optical Microscopy Using Direct Wavefront Measurements
Oscar Azucena, Xiaodong Tao, and Joel Kubby
Index
Author Bio(s)
Joel Kubby is the Department Chair of Electrical Engineering in the Baskin School of Engineering at the University of California at Santa Cruz. His research is in the area of microelectromechanical systems (MEMS) with applications in optics, fluidics, and BioMEMS. Before joining the University of California at Santa Cruz in 2005, he was an area manager with the Wilson Center for Research and Technology and a member of technical staff in the Xerox Research Center Webster in Rochester, New York (1987–2005). Prior to Xerox, he was at the Bell Telephone Laboratories in Murray Hill, New Jersey, working in the area of scanning tunneling microscopy.
Editorial Reviews
"This book is a broad and comprehensive introduction to the use of adaptive optics (AO) in biological microscopy. It provides a much-needed entrée to the field and includes not only the basics and general principles but also discussion of practical implementations and key application areas."
—From the Foreword by Professor Austin Roorda, University of California at Berkeley, and Professor Claire Max, University of California at Santa Cruz
