1st Edition
The Metabolic Pathway Engineering Handbook Fundamentals
This first volume of the Metabolic Pathway Engineering Handbook provides an overview of metabolic pathway engineering with a look towards the future. It discusses cellular metabolism, including transport processes inside the cell and energy generating reactions, as well as rare metabolic conversions. This volume also explores balances and reaction models, the regulation of metabolic pathways, and genome scale and multiscale modeling tools. It also covers developing appropriate hosts for metabolic engineering including the use of Escherichia coli, yeast, Bacillus Subtilis, Streptomyces, filamentous fungi, and mammalian cells using cell culture. Christine Smolke, who recently developed a novel way to churn out large quantities of drugs from genetically modified brewer’s yeast, is regarded as one of the most brilliant new minds in biomedical engineering. In this handbook, she brings together pioneering scientists from dozens of disciplines to provide a complete record of accomplishment in metabolic pathway engineering. With a wealth of cutting edge research and analysis, this work also serves as an invaluable resource for those seeking to add their own contributions. Organized by topic, this 3000 page reference is available as two volumes that can be purchased individually or as a set. For information on the complete handbook visit Cat No. 3923
Volume I
Introduction
and Preface to Handbook, Christina D. Smolke
Progression
of biological synthesis methods towards commercial relevance
The field
of metabolic engineering
An overview
of the metabolic pathway engineering handbook
Metabolic
engineering: looking towards the future
Section I. Cellular Metabolism, Vincent Martin
Transport
processes inside the cell
Energy generating
reactions
Biosynthesis
of important building blocks
Polymerization
of building blocks to macromolecules
Rare
metabolic conversions – harvesting diversity through nature
Section II. Balances and Reaction Models, Walter van Gulik
Growth
nutrients and diversity
Mass
balances, rates, and experiments
Data
reconciliation and error detection
Black box
models for growth and product formation
Metabolic
models for growth and product formation
A
thermodynamic description of microbial growth
Section III. Regulation of Metabolic Pathways, James Liao
Regulation
of specific pathways
Regulating
of secondary metabolism
Building networks as assemblies
of simpler control schemes
Section IV. Modeling Tools for Metabolic Engineering, Costas Maranas
Metabolic
flux analysis
Metabolic
control analysis
Structure
and flux analysis of metabolic networks
Constraint-based
genome-scale models of cellular metabolism
Multi-scale
modeling of metabolic regulation
Validation
of metabolic models
Section V. Developing Appropriate Hosts for Metabolic Engineering, Jens Nielsen
Escherichia coli as a well-developed host for
metabolic engineering
Metabolic
engineering in yeast
Metabolic
engineering in Bacillus subtilis
Metabolic
engineering in Streptomyces
Metabolic
engineering in filamentous fungi
Metabolic
engineering in mammalian cells using cell culture
Biography
Christina Smolke
