1st Edition

Experimental Surgical Models in the Laboratory Rat

Edited By Alfredo Rigalli, Veronica Di Loreto Copyright 2009
    232 Pages 110 B/W Illustrations
    by CRC Press

    An All-Inclusive Guide to Surgical Techniques on Rats

    The design of an adequate surgical model, like the choice of the animal model itself, is extremely important for obtaining reliable valuable data. Experimental Surgical Models in the Laboratory Rat summarizes a series of techniques that were applied in the Bone Biology Laboratories, School of Medicine, Rosario National University, Argentina.

    Stopping just short of an exhaustive analysis of the techniques, this manual describes the basics of each surgical technique, the necessary materials, precautions to consider, the methodology to apply, and the possible results to be obtained in similar experiments.

    CD-ROM: 250 Illustrations of Surgery Techniques with Step-by-Step Instruction

    With succinct descriptions, color photographs, and easy-to-follow steps, the downloadable resources are a practical, full-color roadmap for performing various surgery techniques, such as catheterization, tracheostomy, intramuscular injection, subcutaneous injection, intraperitoneal injection, and oral intubation. This definitive work provides technical and practical data including volumes and concentrations of solutions, catheters used, equipment, and biological variables on the rat. Experimental Surgical Models in the Laboratory Rat is a complete reference for today’s biomedical sciences investigator.

    Section I: Introduction

    Bioethics and Animal Care, Lucas R. M. Brun and Verónica Di Loreto

    Control of Stress and Distress, María F. Landoni

    Management of Pain in Laboratory Animals, María F Landoni

    Anesthesia and Analgesia, Verónica E. Di Loreto, Alfredo Rigalli, and Lucas F. de Candia

    Euthanasia, Alfredo Rigalli

    Antibiotics, Verónica Di Loreto and Alfredo Rigalli

    Section II: General Procedures

    Operating Theatre, Verónica Di Loreto and Alfredo Rigalli

    Rat Identification, Verónica Di Loreto and Alfredo Rigalli

    Antisepsis, Sterilization, and Asepsis, Diego Holotte,- Jésica Nipoti, Maria Julia Pretini, and Verónica Di Loreto

    Suture, Aneley Traverso and Alfredo Rigalli

    Substances Administration, Maela Lupo, Verónica Di Loreto, and Alfredo Rigalli

    Samples, Veronica Di Loreto, Laura Pera, and Alfredo Rigalli

    Section III: Catheterism and Cannulation

    Catheterism of the Femoral Artery and Vein, Verónica Di Loreto and Alfredo Rigalli

    Cardiac Catheterization, Cristina Lorenzo Carrión, Laura Krieger, Manuel Rodríguez, and Martín Donato

    Cannulation of the Thoracic Duct, Gabriel A. Inchauspe and Alfredo Rigalli

    Tracheostomy, Laura L. Pera and Alfredo Rigalli

    Section IV: Gastrointestinal Tract

    In Situ Isolation of the Stomach, Alfredo Rigalli

    In Situ Isolation of the Intestinal Loop, María L. Brance, Lucas R.M. Brun, and Alfredo Rigalli

    Intestinal Everted, Lucas R. M. Brun, María L. Brance, Lucas F. de Candia, and Alfredo Rigalli

    In Situ Perfusion of the Small Intestine, Alfredo Rigalli

    Section V: Pancreas

    Experimental Models for the Study of Diabetes, Verónica Di Loreto and Alfredo Rigalli

    Islets of Langerhans Isolation, Alfredo Rigalli

    Incubation of Pancreatic Tissue Slices, Inés Menoyo and Alfredo Rigalli

    In Situ Perfusion of The Pancreas, Inés Menoyo and Alfredo Rigalli

    Experimental Pancreatitis, Diego Holotte, Jésica Nipoti, Maria J. Pretini, Verónica Di Loreto, and Alfredo Rigalli

    Section VI: Liver

    Hepatic Circulation Impairment, Gabriel A. Inchauspe and Alfredo Rigalli

    Extrahepatic Cholestasis Model, Anabel Brandoni and Adriana M. Torres

    Section VII: Ablation of Endocrine Glands

    Adrenalectomy, Laura Pera and Alfredo Rigalli

    Ovariectomy, Verónica Di Loreto, Laura Pera, and Alfredo Rigalli

    Thyroparathyroidectomy, Verónica Di Loreto and Alfredo Rigalli

    Parathyroid Glands, Maela Lupo and Alfredo Rigalli

    Section VIII: Kidneys

    Measurement Techniques of Renal Parameters, Verónica Di Loreto and Alfredo Rigalli

    Kidney Isolation and Perfusion, Verónica Di Loreto and Alfredo Rigalli

    Acute Renal Failure Models, Gisela Di Giusto and Adriana M. Torres

    Renal Blood Flow Measurement, Gisela Di Giusto, Adriana M. Torres, and Alfredo Rigalli

    Transport Studies in Plasma Membrane Vesicles Isolated from Renal Cortex, Gisela Di Giusto and Adriana M. Torres

    Remnant Kidney Model, María V. Arcidiacono, L. A. Ramirez, V. Dalmau, and Alfredo Rigalli

    Section IX: Circulatory System

    Experimental Myocardial Infarction, Ignacio Seropian and Germán E. González

    Experimental Arteriosclerosis, Anabel Brandoni and Adriana M. Torres

    Isolated Heart: Langendorff Technique, Bruno Buchholz, Verónica D´Annunzio, and Ricardo J. Gelpi

    Section X: Additional Techniques

    Polyclonal Antibodies Preparation, Laura I. Pera, Lucas R. M. Brun, and Alfredo Rigalli

    Histological Procedures, María L. Brance and Lucas R. M. Brun

    Bones and Bone Tissue, Hilda Moreno, Mercedes Lombarte, and Verónica Di Loreto

    Section XI: Miscellaneous

    Equipment, Alfredo Rigalli

    Solutions Preparation, Verónica Di Loreto and Alfredo Rigalli

    Normal Values In Iim/Fm Rat Subline "M", Veronica Di Loreto and Alfredo Rigalli

    Abbreviations

    Biography

    Alfredo Rigalli, Veronica Di Loreto