3rd Edition

Handbook of Molecular and Cellular Methods in Biology and Medicine

    736 Pages 132 B/W Illustrations
    by CRC Press

    Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology. Now in its third edition, this volume provides researchers with an updated tool kit that incorporates conventional as well as modern approaches to tackle biological and medicinal research in the post-genomics era.

    Significantly revised to address these recent changes, the editors have evaluated, revised, and sometimes replaced protocols with more efficient, more reliable, or simpler ones. The book has also been reorganized with section headings focusing on different biological levels connected to one another, taking into account the central dogma of biology (DNA → RNA → protein → metabolites). The book first explores traditional approaches and then moves to the modern "omics" approaches, including genomics, proteomics, and metabolomics. It also discusses the manipulation of biological systems (including RNAi) and macromolecular analyses, focusing on the use of microscopy.

    In each chapter, various notes and cautionary considerations are presented for potentially hazardous reagents. Filled with diagrams, tables, and figures to clarify methods, most chapters also contain Troubleshooting Guides indicating problems, possible causes, and solutions that may be incurred in carrying out the procedures. Researchers and scientists who master the techniques in this book are putting themselves at the cutting edge of biological and medicinal research.

    DNA-Based Technology
    Isolation and Purification of DNA; L. J. Cseke, C. An, and C.-J. Tsai
    DNA Cloning Strategies; L. J. Cseke, G. Trivedi, A. Sreedasyam, P. B. Kaufman, and A. Kirakosyan
    Polymerase Chain Reaction Methodologies; A. Kirakosyan, P. B. Kaufman, L. J. Cseke, and E. M. Seymour
    Preparation of Nucleic Acid Probes; L. J. Cseke and L. R. Cruz-Vera
    Southern Blot Hybridization; C.-J. Tsai and L. J. Cseke
    Genomic DNA Libraries; L. J. Cseke, A. Kirakosyan, and P. B. Kaufman
    DNA Sequencing and Analysis; L. J. Cseke and L. Larka
    RNA-Based Technology
    Isolation and Purification of RNA; C.-Jui Tsai, L. J. Cseke, and S. A. Harding
    RT-PCR and qRT-PCR Methodologies; A. K. Pandey, L. J. Cseke, M. R. Davis, P. B. Kaufman, E. M. Seymour, and A. Kirakosyan
    Preparation of Nucleic Acid Probes; A. Kirakosyan, P. B. Kaufman, and L. J. Cseke
    Northern Blot Hybridization; A. Kirakosyan, P. B. Kaufman, C.-J. Tsai, and L. J. Cseke
    cDNA Libraries; L. J. Cseke, A. Kirakosyan, and P. B. Kaufman
    Differential Display; Y.-S. Wang, S. A. Harding, and C.-Jui Tsai
    Localization of RNA Expression; S. A. Harding, C.-Jui Tsai, and L. J. Cseke
    Isolation and Purification of Proteins; L. J. Cseke, P. B. Kaufman, A. Kirakosyan, and M. V. Westfall
    Western Blot Hybridization; P. B. Kaufman, A. Kirakosyan
    and E. M. Seymour
    Localization of Protein Expression; A. Kirakosyan, P. B. Kaufman, M. V. Westfall, M. Mashore, C. R. Lu, and E. M. Seymour
    DNA Footprinting and Gel Retardation Assay; L. J. Cseke, A. Kirakosyan, and P. B. Kaufman
    Protein–Protein and Protein–Ligand Interactions; M. D. Yoder
    Metabolites
    Isolation and Purification of Metabolites; A. Kirakosyan, K. R. Noon, M. McKenzie, L. J. Cseke, and P. B. Kaufman
    Analysis of Metabolites; K. R. Noon, A. Kirakosyan, M. McKenzie,
    and P. B. Kaufman
    Genomics, Proteomics, and Metabolomics
    Bioinformatics and Data Analysis; S. Holmes and O. De la Cruz Cabrera
    Microarray Platforms; K. P. Venkataramanan, L. J. Cseke, Gopi K. Podila, A. Kirakosyan, and P. B. Kaufman
    Microarray Data Collection; K. P. Venkataramanan, L. J. Cseke, G. K. Podila,
    A. Kirakosyan, and P. B. Kaufman
    Microarray Data Analysis; K. P. Venkataramanan, L. J. Cseke, G. K. Podila, P. B. Kaufman, and A. Kirakosyan
    Proteomics: Data Collection and Analysis; E. H. Kim, D. E. Misek, and M. V. Westfall
    Metabolomics Data Collection and Analysis; T. Okada and A. Katoh
    Common Protocols on Membrane Lipid Analysis and Lipidomics; A. K. Das
    Integrated Functional Genomics; L. J. Cseke, F. Chen, P. B. Kaufman, and A. Kirakosyan
    Manipulation of Biological Systems
    Inhibition of Gene Expression; J. M. Agee, L. Boyd, and L. J. Cseke
    Inhibition of Protein Synthesis; P. B. Kaufman, A. Kirakosyan, and L. J. Cseke
    Gene Transfer and Expression in Animal Cells; S. E. Kampert, E. Devaney, and M. V. Westfall
    Methods of Stem Cell Lineage Analysis In Situ and in Cell Culture; A. B. Borisov
    Tissue Engineering of Fully Differentiated Tissue: Lessons from Work on Cardiac Myocytes; T. K. Stevenson and M. V. Westfall
    Plant Tissue and Cell Culture; N. P. Chanana, L. J. Cseke, A. Kirakosyan, and P. B. Kaufman
    Gene Transfer and Expression in Plants; R. C. Thakur, L. J. Cseke, A. Kirakosyan, and P. B. Kaufman
    Macromolecular Analyses
    Microscopy: Light and Confocal; C. R. Lu, P. B. Kaufman, and L. J. Cseke
    Microscopy: Scanning Electron, Environmental Scanning Electron, and Transmission Electron; C. R. Lu, P. B. Kaufman, A. Kirakosyan, and L. J. Cseke
    Laser Capture Microdissection and Whole Genome Amplification; R. Buyyarapu, V. R. Sripathi, S. B, Cseke, R. V. Kantety
    Index


    Biography

    Leland J. Cseke is an associate research professor in the Department of Biological Sciences at the University of Alabama, Huntsville, where he works in conjunction with the Department of Energy and Argonne National Laboratory to determine the molecular mechanisms of keystone species in forest ecosystem responses to environmental changes. In addition, Dr. Cseke is working with the United States Department of Agriculture to develop cost-effective molecular methods for plant species identification and has helped to develop the national guidelines for plant molecular identification. Other projects include the metabolic engineering of Jatropha curcas plants for the improvement of biodiesel with emphasis on transcriptional regulators that help to control oil biosynthesis.

    Ara Kirakosyan is an associate professor of biology at Yerevan State University, Armenia, and is currently a research scientist at the University of Michigan. Dr. Kirakosyan’s primary research areas include the impact of phytopharmaceuticals on prevention of heart failure, challenges and pitfalls in antioxidant research, and mechanisms of synergistic action of bioactive medicinal compounds at target sites. His research on phytochemicals and antioxidants in foodstuffs has direct implications for the health industry, specifically in the area of cardiovascular disease. He is also interested in plant biotechnology research to produce enhanced levels of medicinally important, value-added secondary metabolites

    Peter B. Kaufman is currently professor emeritus of biology at the University of Michigan, Ann Arbor, and research scientist

    with the University of Michigan Integrative Medicine (UMIM) program. He is a Fellow of the American Association for the Advancement of Science (AAAS). He is also past president of the Michigan Botanical Club and past chairman of the Michigan Natural Areas Council. He served as secretary-treasurer of the American Society of Gravitational and Space Biology (ASGSB) and was the recipient of ASGSB’s Orr Reynolds Distinguished Service Award. He is currently doing research on natural products of medicinal value in plants with support from UMIM, the National Institutes of Health, Xylomed Research, Denali BioTechnologies, LLC, and the Cherry Marketing Institute (CMI) of Michigan.

    Margaret V. Westfall is an associate professor of surgery and molecular and integrative physiology and an assistant professor of biomedical engineering at the University of Michigan, Ann Arbor, Michigan. Her research is focused on heart failure and includes studies on protein kinase C modulation of contractile function; the role of the thin filament molecular switch protein, troponin I, in modulating contractile function; and analysis of proteomic expression in end stage heart failure. Dr. Westfall’s research has been funded by the National Institutes of Health and the American Heart Association. She currently serves on the editorial board for the American Journal of Physiology—Heart and Circulatory Physiology and the Journal of Molecular and Cellular Cardiology.