The Evolution from Protein Chemistry to Proteomics: Basic Science to Clinical Application
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Features
Includes a detailed discussion of the rationale for the use (and misuse) of protease inhibitors in sample preparation
Discusses issues concerning the application of clinical chemistry essentials, such as the index of individuality
Merges current concepts of proteomics with the existing literature in solution protein chemistry
Reviews areas of proteomics research that are critical to assay validation
Discusses clinical applications of proteomics, with an emphasis on the development of diagnostic assays
Summary
Largely driven by major improvements in the analytical capability of mass spectrometry, proteomics is being applied to broader areas of experimental biology, ranging from oncology research to plant biology to environmental health. However, while it has already eclipsed solution protein chemistry as a discipline, it is still essentially an extension of classical protein chemistry, owing much of its maturation to prior contributions. Unfortunately, this debt is not always evident in current literature.
The Evolution from Protein Chemistry to Proteomics: Basic Science to Clinical Application, in providing a different perspective than other reviews, strengthens the connection between solution protein chemistry and proteomic technology. Towards this end, Roger Lundblad, a long-time leader in protein chemistry and a scientist who has worked in both academics and industry, brings together some seemingly disparate areas into a single volume. Discussing analytical proteomics, expression proteomics, and clinical proteomics (biomarker identification), he provides coverage that is uniquely rich in detail.
Lundblad applies this detail to sample preparation for proteomic analysis, including preparation from blood and tissues. He also presents specifics on the prefractionation of samples used to identify specific subproteomes such as phosphoproteomes and glycoproteomes. Comprehensive reviews are provided covering the chemical modification of proteins, including its use for chemical proteomics. Special attention is given to challenges that impede the identification, validation, and development of biomarkers into clinically useful diagnostic analytes.
A bestselling author, Lundblad utilizes classical protein chemistry literature in providing an intellectual basis for proteomics that merges current concepts with the existing literature, while providing the technical detail necessary for the effective commercialization of proteomics.
Table of Contents
A Brief Discussion of Proteomics – Definitions – Concepts – Illusions
An Overview of the Chemical Modification of Proteins
The Application of Chemical Modification to Proteomics – Chemical Proteomics
Sample Preparation for Proteomic Studies
Sample Prefractionation
An Overview of Analytical Technologies Used in Proteomic Research
Clinical Proteomics
Validation Issues in Proteomics and the Transition from Research to Diagnostic Development
References
Editorial Reviews
“It is a rainbow of protein reactions with all possible chemicals you could dream of, whose reaction schemes and formulae are hardly found in any of the books devoted to proteomics. Lundblad has resurrected the rigorous chemistry literature of the 1950’s and 60’s, on which most of our present-day knowledge is based.”
—Proteomics, Vol. 6, No. 7, April 2006
“This book is of great value to all research scientists and students with interests in all areas of proteomic science and protein chemistry.”
—John F. Kennedy in International Journal of Biological Macromolecules, September 2006
“This book, in strengthening the connection between solution protein chemistry and proteomic technology, provides a different perspective than others reviews. … provides coverage that is uniquely rich in detail. … also presents specifics on the prefractionation of samples and provides comprehensive reviews covering the chemical modification of proteins, leading up to and including its use in proteomics.”
— In Anticancer Research, Vol. 26, 2006
"Dr. Roger L. Lundblad, a recognized expert in protein chemistry, is well known for his contributions to the chemical medication of proteins . . . With his extensive expertise in protein chemistry, Dr. Lundblad is in a privileged position to outline the evolution of the field . . . In summary, this may be a useful book for readers already familiar with the essential aspects of proteomics. In stressing the connection, both historical and current, between classical protein chemical modification and modern proteomics methodologies, Dr. Lundblad is undoubtedly on target, and this is clearly the strongest point of the book."
– Dr. David Andreu, Pompeu Fabra University, in ChemMedChem, 2008, No. 3
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