1st Edition

Cell Electrophoresis

By Johann Bauer Copyright 1994

    This book presents a summary of the application and instrumentation of cell electrophoresis. The method of making cell purification and characterization possible according to the cellular negative surface charge density is discussed, and ideas for future developments are explained. The negative electrostatic forces at cell surfaces provide information about cell-cell interaction, blood vessel sealing, cytokine actions, cell transformation, ion transport phenomena, and other biological phenomena. Recalculations of the physical principles of cell electrophoresis reveal possibilities for removing disruptive factors caused by electrical current, heat, and sedimentation. The introduction of computer technology, the performance of simultaneous two-parameter measurements, and the application of cell-friendly but current-inert buffer systems render the method more reliable and efficient.

    Theoretical Considerations:
    Contributions to a Mathematical Theory of Free Flow Electrophoresis, F.G. Boese
    Numerical Description of Zone Electrophoresis in the CFE Device, T.M. Grateful, A.M. Athalye, and E.N. Lightfoot
    Improvements of the Method:
    Theoretical and Experimental Studies on the Stabilization of Hydrodynamic Flow in Free-Fluid Electrophoresis, P.H. Rhodes and R.S. Snyder
    The Loading and Unloading of Cells in Electrophoretic Separations, P. Todd
    Electrode Compartment Separating Membranes for Cell Electrophoresis, J. Heinrich and H. Wagner
    Cell Electrophoresis Using Antibodies and Antigens as Ligands, H.H.P. Cohly and S.K. Das
    Buffers and Their Effects on Cells:
    New Buffer Systems for Cell Electrophoresis, K.A. Kinsley and L.S. Rodkey
    Alteration of Cellular Features after Exposure to Low Ionic Strength Medium, I. Bernhardt
    Electrophoresis of Cells at Physiologic Ionic Strength, M.V. Golovanov
    The Importance of Determination of the Negative Surface Charge Density of Cells:
    Simultaneous Two-Parameter Measurements of the Electrophoretic Features of Cell Populations and Their Sedimental Characteristics, G.G. Slivinsky
    Cell Interactions and Surface Hydrophilicity: Influence of Lewis Acid-Base and Electrostatic Forces, C. J. van Oss
    Electrical Surface Phenomena of Endothelial Cells, F.F. Vargas
    Application of Cell Electrophoresis for Clinical Diagnosis, W. Schütt, N. Hashimoto, and M. Shimizu
    The Negative Surface Charge Density of Cells and Their Actual State of Differentiation or Activation, J. Bauer
    Cell Electrophoresis in Space:
    Cell Electrophoresis in Microgravity: Past and Future, D.R. Morrison
    Cell Electrophoresis in Microgravity, U. Friedrich, G. Ruyters, and J. Bauer

    Biography

    Johann Bauer