This book describes, in detail, tested techniques for the produc-tion and use of monoclonal antibodies. It covers those aspects of interest to all scientists working with monoclonal antibodies and presents methods in a step-by-step format for easy refer-ence. The text serves as a laboratory manual; and discusses rationale behind each method, and the choices between methods. It also provides a rational basis where several alternative methods are available.
Introduction. Overview. Making Hy-bridomas. Monoclonal Antibodies: Production, Purification, Analysis, Quality Control, Storage, and Distribution. Using Monoclonal Antibodies: Cell and Tissue Markers. Using Monoclonal Antibodies: Soluble Antigens. Prospects, Problems, and Limitations in the Use of Monoclonal Antibodies. Appendixes.