Making and Using Antibodies: A Practical Handbook

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  • Describes basic laboratory techniques in detail, accompanied by practical examples
  • Contains an outstanding selection of references for follow-up work
  • Includes cross-referencing of methods allowing for easy access to information
  • Illustrates the importance of ethical considerations and safety in laboratory research
  • Features introductory material for each chapter, providing a better understanding of the methods and applications
  • Examines potential problems and notes trouble-shooting tips
  • Summary

    Antibodies are an indispensable tool in the study of biology and medicine. Making and Using Antibodies: A Practical Handbook presents techniques in a single, comprehensive source for the production and use of antibodies. It enables researchers to immediately access lab-tested, proven protocols.

    Written and edited by an elite team of scientists, who have developed and refined many of the methods, this book covers-

  • Commercially available adjuvants designed for the production of antisera in the research setting
  • Methods for the production, purification, and characterization of antibodies
  • Practical guidance to researchers needing to modify antibodies
  • Basic techniques including enzyme-linked immunosorbent assay (ELISA), Western blotting, immunohistochemistry, and flow cytometry
  • Methods for applying immunolabeling to the realm of electron microscopy (EM)
  • New antibody sources and new ways to use antobodies

    Making and Using Antibodies: A Practical Handbook also offers invaluable insight into future directions, challenges, and opportunities both in research and industrial applications.
  • Table of Contents

    Antibodies; Matthew R. Kaser and Gary C. Howard

    Antigens; Paul Algate, Jory Baldridge, and Sally Mossman

    Adjuvants; Jory Baldridge, Paul Algate, and Sally Mossman

    Production of Polyclonal Antibodies; Lon V. Kendall

    Production of Monoclonal Antibodies; Kathleen C. F. Sheehan

    Quantitative Production of Monoclonal Antibodies; David A. Fox and Elizabeth M. Smith

    Purification and Characterization of Antibodies; Joseph P.

    Making Antibodies in Bacteria; Frederic A. Fellouse and Sachdev S. Sidhu

    Chemical and Proteolytic Modification of Antibodies; George P. Smith

    Applications; Lee Bendickson and Marit Nilsen-Hamilton

    Immunohistochemical Methods; José A. Ramos-Vara and Julie Ackerman Saettele

    Immunoelectron Microscopy; Sara E. Miller and David N. Howell

    Flow Cytometry; Kristi R. Harkins and M. Elaine Kunze

    ELISAs; John Chen and Gary C. Howard

    Antibodies in the Future: Challenges and Opportunities; Matthew R. Kaser and Gary C. Howard