Contains user-friendly, step-by-step protocols applicable to a wide range of research needs

Provides examples of anticipated results

Outlines pitfalls and limitations of the techniques and methods described

Includes information on materials and resources needed for each technique

Notes recent trends such as government and industry promotion of molecular medicine and integration of neuroscience with cellular and molecular research techniques

Arousal states are processes that include waking, deep sleep, and the dreaming phase of sleep (REM). Molecular Regulation of Arousal States explores the cellular and molecular mechanisms by which sleep and wakefulness are regulated and seeks explanations for the generation of arousal states. It presents step-by-step research protocols that allow investigators to apply the techniques described to a wide range of physiological and behavioral research problems, such as sleep neurobiology and state-dependent disruption of cardiopulmonary control. For the first time, a single source integrates cellular and molecular research techniques with studies of arousal, opening the door to exciting new research methodologies.

Application of In Situ Hybridization to the Study of Rhythmic Neural Systems, H.E. Albers, R.T. Zoeller, and K.L. Huhman
Estimation of the mRNAs Encoding the Cholinergic Muscarinic Receptor and Acetylcholine Vesicular Transport Proteins Involved in Central Cardiovascular Regulation, J.J. Buccafusco, L.C. Zhang, and M.A. Prendergast
Voltammetric Detection of Nitric Oxide (NO) in the Rat Brain: Release Throughout the Sleep-Wake Cycle, S. Burlet and R. Cespuglio
Sleep Regulatory Substances: Change in mRNA Expression Linked to Sleep, Z. Chen and J.M. Krueger
Immediate Early Genes as a Tool to Understand the Regulation of the Sleep-Waking Cycle: Immunocytochemistry, In Situ Hybridization, and Antisense Approaches, C. Cirelli, M. Pompeiano, and G. Tononi
Methods for the Measurement of Adenylyl Cyclase Activity, C.W. Emala
Methods Used to Assess Specific Messenger RNA Expression During Sleep, M.A. Greco, L. Ramanathan, R. Basheer, and P.J. Shiromani
Competition Binding Assays for Determining the Affinity and Number of Muscarinic Receptor Subtypes in Tissue Homogenates, A.U. Höglund and H.A. Baghdoyan
Isolation and Identification of Specific Transcripts by Subtractive Hybridization, T.S. Kilduff, L. de Lecea, H. Usui, and J.G. Sutcliffe
Use of In Situ Hybridization Histochemistry to Study Muscarinic Receptor mRNA Expression in Brains of Sleep-Deprived Rats, C. Kushida and D.M. Simmons
Transcriptional Regulation of Putative Sleep Promoting Compounds, M. Mackiewicz, S.C. Veasey, and A.I. Pack
Chemical Mutagenesis and Screening for Mouse Mutations with an Altered Rest-Activity Pattern, P.M. Nolan, T.A. Houpt, and M. Bucan
Reverse Transcription mRNA Differential Display: A Systematic Molecular Approach to Identify Changes in Gene Expression Across the Sleep-Waking Cycle, M. Pompeiano, C. Cirelli, and G. Tononi
In Situ Hybridization of Messenger RNA in Sleep Research, T. Porkka-Heiskanen, J. Toppila, and D. Stenberg
New Directions in the Analysis of Brain Substances Related to Sleep and Wakefulness, G. Siuzdak and S. Henriksen
Sleep and Circadian Rest-Activity Rhythms in Prion Protein Knockout Mice, I.M. Tobler, M. Fischer, and J. Manson
Measurement of Nitric Oxide in the Brain Using the Hemoglobin Trapping Technique Coupled with In Vivo Microdialysis, J.A. Williams, S.R. Vincent, and P.B. Reiner
Mapping Regional Cerebral Protein Synthesis During Sleep, R.K. Zoltoski
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