Methods in Genomic Neuroscience

Series:
Frontiers in Neuroscience
Published:
September 26, 2001 by CRC Press - 344 Pages
Editor(s):
Hemin R. Chin, National Eye Institute/NIH, Bethesda, Maryland, USA; Steven O. Moldin, University of Southern California, Washington, DC, USA

Purchasing Options

Hardback
$169.95
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ISBN 9780849323973
Cat# 2397
 

Features

  • Discusses state-of-the-art methods used to characterize the genetic basis of the nervous system and complex behavior
  • Presents clearly written chapters, making each methodology accessible to neuroscientists with different levels of experience and expertise in molecular techniques
  • Provides researchers with detailed information for choosing and implementing the appropriate genetic methodology for their application

    • Summary

    The past few years have witnessed extraordinary advances in molecular genetic techniques and the accumulation of structural genomics information and resources in both human and model organisms. With the development of new technologies and the availability of resources like the sequence of eukaryotic genomes, problems of a previously unthinkable scope are now being routinely solved in neuroscience and many other areas of biomedical research. The results of these studies, in turn, are having, and will continue to have, profound impact on experimental approaches and designs for manipulating genes, the genome, and model organisms as a means of gaining insights into nervous system functioning and complex behavior.

    Methods in Genomic Neuroscience provides newcomers and experienced researchers with a reference guide for applying powerful, state-of-the-art molecular genetic techniques to the study of neural and behavioral systems. It thereby provides a foundation by which data on gene expression and function may be used to develop new therapeutic strategies for brain diseases.

    Authored by researchers in the forefront of genomic neuroscience, the book addresses state-of-the-art tools and technologies for global analysis of genes influencing the nervous system and its disorders. An emphasis is placed on massively parallel approaches for analyzing the avalanche of data that is being generated from the full genomic sequence of humans and model systems. Specific topics include human sequence variation, methods for discovering disease vulnerability genes, gene-environmental interaction, gene expression analysis using DNA microarrays, random mutagenesis, gene trap approaches for studying brain development, neural stem cells, gene targeting, and gene delivery.

    Table of Contents

    PREFACE

    FORWARD
    Floyd E. Bloom, M.D.

    Gene-Environment Interaction and Complex Behavior
    Marla B. Sokolowski, Ph.D. and Douglas Wahlsten, Ph.D.

    Current Perspectives on the Genetic Analysis of Naturally-Occurring Neurological Mutations in Mice
    Wayne N. Frankel, Ph.D.

    Conditional and Inducible Gene Targeting in the Nervous System
    Mark Mayford, Ph.D. and Eric Kandel, M.D.

    Functional Identification of Neural Genes
    Lawrence H. Pinto, Ph.D. and Joseph S. Takahashi, Ph.D.

    Studying Brain Development and Wiring Using a Modified Gene Trap Approach
    Kevin J. Mitchell, Ph.D., Lisa V. Goodrich, Ph.D., Philip A. Leighton, Ph.D., Xiaowei Lu, Ph.D., Kathy Pinson, Ph.D., Paul Scherz, Olivia G. Kelly, Ph.D., Joel Zupicich, Paul Wakenight, Peri Tate, Ph.D., Judy Mak, Edivinia Pangilinan, Helen Rayburn, Ph.D., Danielle Rottkamp, Joe Zhong, William C. Skarnes, Ph.D., and Marc Tessier-Lavigne, Ph.D

    N-Ethyl-N-Nitrosourea (ENU) Mutagenesis: Functional Analysis of the Mouse Nervous System and Behavior
    Stephen Kanes, M.D., Ph.D., Gillian R. Leach, and Maja Bucan, Ph.D.

    DNA Arrays and Gene Expression Analysis in the Brain
    David J. Lockhart, Ph.D. and Carrolee Barlow, M.D., Ph.D.

    DNA Microarrays and Human Brain Disorders
    Karoly Mirnics, M.D., David A. Lewis, M.D., and Pat Levitt, Ph.D.

    Full-Length cDNA Libraries: Reagents for Functional Studies of the Nervous System
    Munetomo Hida, Ph.D., Yutaka Suzuki, Ph.D., and Sumio Sugano, M.D.

    Methods for Gene Delivery to Neural Tissue
    Jürgen A. Hampl, M.D., Alice B. Brown, M.D., Ph.D., Nikolai G. Rainov, M.D., and Xandra O. Breakefield, Ph.D.

    Neural Stem Cells and Brain Repair
    Lorenz Studer, M.D. and Ron McKay, Ph.D.

    Statistical Methods to Discover Susceptibility Genes for Nervous System Diseases
    Jurg Ott, Ph.D.

    Genetic Variation Analysis of Neuropsychiatric Traits
    Michael E. Zwick, Ph.D., David J. Cutler, Ph.D., Aravinda Chakravarti, Ph.D.

    Editorial Reviews

    "It is primarily an excellent state-of-the-art overview of mouse neurogenetics, covering advanced methods of genetic manipulation and analysis in forward and reverse genetics.... Each of these practitioners and facilitators of modern neuroscience will benefit from reading this book... I recommend reading Methods in Genomic Neuroscience in its entirety - from start to finish, or from finish to start, or in any order. That is a compliment that one can rarely offer a book of the methods type."
    -Nature Neuroscience, November 2001

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