DNA Arrays: Technologies and Experimental Strategies
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Summary
A quantum leap in technology took place a few years ago with the introduction of cDNA arrays that have been developed in response to the need for simultaneous analysis of the patterns of expression of thousands of genes. DNA Arrays: Technologies and Experimental Strategies offers a view of different aspects of this rapidly developing technology, including oligonucleotide and electrochemical arrays. Distinguished investigators from academia and industry provide up-to-date insights into methodological fundamentals of construction of DNA arrays, optimization of hybridization conditions, the use of expression data in bioinformatics, software tools, and the application of microarray technology for drug discovery.
Table of Contents
Introduction
Technology Development for DNA Chips, H. Eickhoff, U. Schneider, E. Nordoff, L. Nyarsik, G. Zehetner, W. Nietfried, and G. Lehrach
Experimental Design for Hybridization Aray Analysis of Gene Expression, W. Freeman and K. Vrana
Nylon Platform: Pros and Cons, E. Grigorenko, S. Weber
Oligonucleotide Array Technologies for Gene Expression Profiling, D. Doris, R. Ramakrishnan, T. Sendera, S. Magnuson, and A. Mazumder
Electrochemical Detection of Nucleic Acids, R. Witwer, E. Espenhahn, M. Napier, and H. Thorp
DNA Microarrays in Neurobiology, M-C. Potier, B. Golfier, N. Cauli, B. Gibelin, B. Le Bourdelles, B. Lambolez, S. Kuhlmann, P. Marc, F. Devaux, and J. Rossier
Data Management in Microarray Fabrication, Image Processing and Data Mining, A. Kuklin, S. Shah, B. Hoff, and S. Shams
Zeroing In on Essential Gene Expression Data, S. Fuhrman, S. Liang, X. Wen, and R. Somogyi
Application of Arrayed Libraries for Analysis of Differential Gene Expression Followed Chronic Cannabinoid Exposure, E. Grigorenko, J. Kittler, and S. Deadwyler
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