Cell-Penetrating Peptides: Processes and Applications
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Summary
One of the major problems with drug delivery is the inability of large hydrophilic compounds to pass through the lipid membrane of the cell, thus making drugs such as polypeptides and oligonucleotides of limited therapeutic value. Until recently, the transport of such molecules into the cytoplasmic and nuclear compartments of living cells seemed a far off goal. Hence, the discovery that peptides derived from the HIV Tat protein and homeodomain of the Antennapedia transcription factor (penetratin) can translocate across the plasma membrane of live cells has opened up exciting new possibilities in biomedical research.
This book fills the need for a comprehensive update of the research concerning these specialized peptides, called cell-penetrating peptides (CPPs), to which the drug molecule can attach and pass through the cell membrane. Divided into three parts, Cell-Penetrating Peptides: Processes and Applications covers classes of cell-penetrating peptides; mechanisms of cell penetration, interactions of cell-penetrating peptides with plasma membranes, and lipid models; and applications of cell-penetrating peptides. These divisions cover all information from basic biochemistry to clinical application, including a clear description of the general rules for cellular uptake of the transport peptides. The overview of work in this area will lead to better designed novel transport peptides to achieve the more successful application of CPPs in research and therapy.
Table of Contents
Preface, Ülo Langel
CLASSES OF CELL-PENETRATING PEPTIDES
The Tat-Derived Cell-Penetrating Peptide, Eric Vivès and Bernard Lebleu
Penetratins , Edmond Dupont, Alain Joliot and Alain Prochiantz
Transportans, Margus Pooga, Mattias Hällbrink and Ülo Langel
MAP Peptides, Johannes Oehlke, Burkhard Wiesner and Michael Bienert
Signal Sequence-Based Cell-Penetrating Peptides (NLS) and Their Applications for Gene Delivery, May Morris, Laurent Chaloin, Frédéric Heitz and Gilles Divita
Hydrophobic Membrane Translocating Sequence (MTS) Peptides, Kristen Sadler, Yao-Zhong Lin and James P. Tam
Arginine-Rich Molecular Transporters for Drugs: The Role of Backbone and Sidechain Variations on Cellular Uptake, Jonathan B. Rothbard, Erik Kreider, Kanaka Pattabiraman, Erin T. Pelkey, Christopher L. VanDeusen, Lee Wright, Bryan L. Wylie and Paul A. Wender
MECHANISMS OF CELL PENETRATION, INTERACTIONS OF CELL-PENETRATING PEPTIDES WITH PLASMA MEMBRANES AND LIPID MODELS
Interactions of Cell-Penetrating Peptides with Membranes, Laurent Chaloin, Nicole Van Mau, Gilles Divita and Frédéric Heitz
Structure Prediction of CPPs and Iterative Development of Novel CPPs, Olivier Bouffioux, Frédéric Basyn, René Rezsohazy and Robert Brasseur
Biophysical Studies of Cell-Penetrating Peptides, Astrid Gräslund and L.E.G.Eriksson
Toxicity and Side-Effects of Cell-Penetrating Peptides, Margus Pooga, Anna Elmquist and Ülo Langel
Quantification of Cell-Penetrating Peptides and Cargoes, Maria Lindgren, Mattias Hällbrink and Ülo Langel
Kinetics of Uptake of Cell-Penetrating Peptides, Matjaz Zorko, Mattias Hällbrink and Ülo Langel
Signal Peptides, Kenta Nakai
APPLICATIONS OF CELL-PENETRATING PEPTIDES
Cell-Penetrating Peptide Conjugations and Magnetic Cell Labels, Ching-Hsuan Tung and Ralph Weissleder
Cell-Penetrating Peptides as Vectors for Delivery of Nucleic Acids, Andres Valkna, Ursel Soomets and Ülo Langel
Protein Transport, Jay S. Wadia, Michelle Becker-Hapak and Steven F. Dowdy
Microbial Membrane Permeating Peptides and Their Applications, K.R. Gunaratna, Mats Andersson and Liam Good
