Methods in Cell Wall Cytochemistry
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Summary
Various methodologies designed to study cell walls are compiled in this book.
Methods in Cell Wall Cytochemistry covers the use of modern dyes, fluorescent chemicals, lectins, and antibody technology (immunocytochemisty.)
Cell wall morphology and chemical composition is covered as well as light and fluorescent cytochemistry; transmission electron microscopic cytochemistry; lectin cytochemistry; and, special emphasis on immunocytochemistry.
Addressing an emerging area of research and technology, this book will appeal to plant pathologists, cell biologists, as well as workers interested in stress response and those employing cell walls for biotechnological research.
Table of Contents
Preface
Cell Wall-Morphology & Chemical Composition
Carbohydrates
Cellulose, Callose and other 1, 3-b-glucans, Chitins, Pectins, Hemicelluloses, Alginic Acid, Sulphated Polysaccharides
Glycoproteins
Structural Proteins-Lectins, Arabinogalacton Rich Proteins (AGP's), Hydroxy Proline Rich Proteins, Proline Rich Proteins, Glycine Rich Proteins, Threonine Rich Proteins, Thionins, Pistil Specific Extensin-Like Proteins
Enzymes-Peroxidase, Polyamineoxidase, Phosphatase, Protease, Nitrate Reductase, Malate Dehydrogenase, Proline Hydroxylase, Glycosylhydrolases and Transferases, Non-Specific Esterases, and Ribonucleuses
Lipids and Related Substances-Cutin, Suberin, Waxes, Sporopollenin, Pollenkitt, and Tryphine
Lignin and Other Phenolic Substances-Lignins, Phenolic Acids and Amides
Mineral Substances-Silica, Calcium, and Boron
Light Microscopic Cytochemistry
Introduction
Tissue Preparation-Use of Fresh Living Tissue, Use of Frozen Tissue, Freeze Substitution and Drying, Chemical Fixation, Dehydration and Clearing, Infiltration and Embedding, Sectioning of Materials
Stains and Staining-Introduction, Control Procedures, Incubation of Materials
Mounting of Stained Materials
Procedures in Tissue Preparation for Light Microscopic Cytochemistry-Freezing, Freeze Substitution and Drying Methods, Preparation of Fixatives, Dehydration and Clearing Procedures
Procedures for the Cytochemical Localizations of Different Cell Wall Substances
Carbohydrates-Insoluble Polysaccharides, Cellulose, Chitin, Callose, Sulphated and Carboxylated polysaccharides
Proteins-Non-Specific Cell Wall Proteins, Enzymic Proteins
Lignins
Sporopollenins
Cutins & Suberin
Minerals
Fluorescent Microscopic Cytochemistry
Introduction
Fluorescent Cytochemical Methods
Cell Wall Carbohydrates
Cell Wall Proteins
Lignin
Ferulic Acid
Sporopollenin
Cutin and Suberin
Transmission Electron Microscopic Cytochemistry
Introduction
Specimen Preparation
Electron Microscopic Staining
TEM Cytochemical Methods
Cell Wall Carbohydrates
Cell Wall Proteins
Cutin and Suberin
Lectin Cytochemistry
Introduction
Procedures Involving Lectin Probes
Immunocytochemistry
Introduction
Basics of Immunocytochemistry-Immunization,
Storing and Purifying Antibodies, Labeling of Antibodies, Tissue Preparation, Immunolabeling, Mounting of Sections
Methods in Immunocytochemistry-Methods in Antibody Production and Tissue Preparation, Application of Immunocytochemistry to Study Cell Wall Components
Literature Cited (at the end of each Chapter)
