Delineating its usage in separation, purification and detection processes across a variety of disciplines, from industry to applied research, this work discusses the principles, techniques and instrumentation involving HPLC within a detailed framework. Over 100 tables present previously scattered experimental data.

Fundamentals: retention and selectivity; the mechanisms and importance of zone-spreading; principles of detection; capillary electrophoresis; programmed analysis; computers and liquid chromatography. HPLC techniques: size exclusion chromatography; reversed-phase stationary phases; normal-phase liquid chromatography; HPLC of ions - ion-exchange chromatography; ion chromatography by HPLC; hydrophobic interaction chromatography of biopolymers; affinity chromatography. HPLC instrumentation: mobile-phase delivery systems for HPLC; liquid chromatography detectors; injection devices; tandem liquid chromatography systems; temperature control in analytical high-performance liquid; chromatography; collection devices. HPLC applications: HPLC application of drugs in biological samples; HPLC applications for chiral pharmaceutical analysis; HPLC applications in biotechnology; HPLC applications in food and nutritional analysis; HPLC analysis of surfactants; HPLC applications to the analysis of ions and inorganic species; HPLC applications to polymer analysis; HPLC applications in physicochemical measurements; HPLC applications in art conservation; sample handling and analysis of organic pollutants (pesticides and phenols) in water matrices by HPLC.

Editorial Reviews

". . .provides a comprehensive overview of HPLC fundamentals, techniques, instrumentation and applications in one volume. . .. . . .well structured and well presented. It is certainly eminently suitable as a textbook for undergraduate students. . ..also suitable for a side range of HPLC practitioners--from new users who want a better understanding of the technique through to the old hands' who want to catch up on the latest innovations. "
---Applied Organometallic Chemistry Journal
"The editors. . ..should be congratulated as they have provided a book which is an invaluable introduction to the beginner but at the same time is an indispensable reference work for the experienced chromatographer. It is highly recommended for inclusion in personal and laboratory libraries. "
---Bioseparation