Optical Imaging Techniques in Cell Biology
Editor(s):  Guy Cox
Related Titles
In Vivo Optical Imaging of Brain Function, Second Edition
Editor(s):  Ron Frostig, University of California, Irvine, Irvine, California, USA
Publication Date: May 06, 2009
Price: $149.95
The Image Processing Handbook, Fifth Edition
John C. Russ, North Carolina State University, Raleigh, USA; John C. Russ, North Carolina State University, Raleigh, USA
Publication Date: December 19, 2006
Price: $173.95
Scanning Probe Microscopes: Applications in Science and Technology
K. S. Birdi, Consultant, Charlottenlund, Denmark
Publication Date: February 26, 2003
Price: $194.95
Virtual Microscopy and Virtual Slides in Teaching, Diagnosis, and Research
Editor(s):  Robert W. Ogilvie, Medical University of South Carolina, Charleston, USA
Publication Date: June 22, 2005
Price: $184.95
Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition
Editor(s):  Leland J. Cseke, The University of Alabama, Huntsville, USA;  Peter B. Kaufman, University of Michigan, Ann Arbor, USA;  Gopi K. Podila, University of Alabama, Huntsville, USA;  Chung-Jui Tsai, Michigan Technological University, Houghton, USA
Publication Date: November 24, 2003
Price: $173.95
Atlas of Cell Organelles Fluorescence
Elli Kohen, Miami, Florida, USA; Rene Santus, Museum National d'Histoire Naturelle, Paris, France; Joseph G. Hirschberg, University of Miami, Coral Gables, Florida, USA; Nuri Ozkutuk, Celal Bayar University, Manisa, Turkey
Publication Date: December 29, 2003
Price: $199.95
Price:  $152.95
Cat. #:  3919
ISBN:  9780849339196
ISBN 10:  0849339197
Publication Date:  November 06, 2006
Number of Pages:  288
Availability:  In Stock
Binding(s):  Hardback | Available in e-book!

Email this title to a friend
Description
Table of Contents
Reviews
Features
  • Provides an understanding of the underlying principles, benefits, and limitations of a wide range of optical techniques currently used in cell biology
  • Presents a cogent, sophisticated discussion without relying on complex mathematics
  • Includes multiple appendices on practical maintenance, cell handling, labeling, and image manipulation
  • Draws from the teaching and research experience of an internationally known figure in the field
  • Employs line drawings and color photographs to illustrate the principles discussed

    • Summary
    Since the word microscopy was coined in 1656, the evolution of the instrument has had a long and convoluted history. Plagued with problems of chromatic aberration, spherical aberration, and challenges with illumination and resolution, the microscope’s technical progression happened in a series of fits and starts until the late 19th century. After Ernst Abbe perfected the “how” of lens design, achieving the theoretical limit imposed by wavelength, there came a revolution in subject matter or “what” could be studied by microscope.
    Covering the entire field of microscopy, Optical Imaging Techniques in Cell Biology provides an overview of the technical evolution of the microscope and explains how the basics of optical microscopy led to the most advanced techniques employed today. The author addresses a vast array of topics including optical contrasting techniques, fluorescence, confocal versus widefield microscopes, lasers as a light source, and digital imaging, as well as the correction of aberrations that might arise. Building on this foundation, he then examines more advanced techniques such as quantitative fluorescence, fluorescence resonant energy, three-dimensional imaging, high-speed confocal microscopy, non-linear microscopy, and stimulated emission depletion.
    Delivering a truly comprehensive work encompassing the scope and breadth of the field, the author brings a new level of understanding to the student, technician, researcher, or investigator working in the fascinating realm of optical microscopy.